Intensification Starts Here!

Discover how KrosFlo® TFDF® based process intensification leads to high virus production combined with simplified clarification compared to batch cell culture.

  • Implement TFDF® to mitigate cell density effects
  • TFDF® facilitates intensified production of lentivirus and AAV
  • Simplify clarification techniques to streamline your production process

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  INNOVATION BREAKTHROUGH

TF + DF = TFDF® Combined Benefits

Tangential Flow

High cell density
Poor product passage

Depth Filtration

Low cell density
Excellent product passage

Tangential Flow Depth Filtration

High cell density
Excellent product passage

  INNOVATION BREAKTHROUGH

Novel TFDF® Filter 

The thick-walled 2 - 5 µM tubular filter captures cells and cell debris with reduced fouling and negligible product retention. The feed stream enters the bottom port. The retentate travels through the lumen and exits at the top of the tube. The permeate exits the enclosure through the lower permeate port.​solution. The combination of tangential flow and depth filtration in one filter produces a high-flux, high-capacity solution.  h

  INNOVATION BREAKTHROUGH

Synergistic Benefits

  • Low concentration polarization minimizes fouling
  • Short, highly inter-connected paths do not retain product​
  • Small footprint
  • 2-5 micron pore size for large biomolecules
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  INTEGRATED

Filter, Flow Path and System as One Solution

KrosFlo® TFDF® Filter
ProConnex® TFDF® Filter Assembly
KrosFlo® TFDF® Systems
  INTEGRATED

Complete, Ready-to-use TFDF® Filter Assemblies

Single-use, Gamma-irradiated and closed ProConnex® TFDF® Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state. ProConnex® TFDF® Flow Paths are complete assemblies that include the TFDF® Filter, a disposable pump head​, integrated sensors​, tubing​ and aseptic connectors.

TFDF® Filter
Surface Area: 3 cm2
Surface Area: 30 cm2

ProConnex® TFDF®
Flow Path
Surface Area: 3 cm2
Surface Area: 30 cm2
Surface Area: 50 cm2

ProConnex® TFDF® 
Flow Path

Surface Area: 150 cm2

ProConnex® TFDF® 
Flow Path

Surface Area: 450 cm2
Surface Area: 750 cm2
Surface Area: 1500 cm2
Surface Area: 2100 cm2

ProConnex® TFDF®
Flow Path
Surface Area: 6000 cm2

Industrial grade components and extended automation allow for confident process scaling. With the same software, hardware, controls and cGMP compliance built into every system, process change challenges in scale-up are removed.

READY TO SCALE FAST

Maintain processes while scaling to production

The KrosFlo® TFDF® System delivers application-specific process control logic for optimal TFDF process performance. Systems scale from 1 L to 2000 L in a small footprint of 1 m2 for both pilot (31 inch x 42 inch) and process (37 inch x 42 inch) systems.

KrosFlo® TFDF Lab System

1 - 50 L volume
3 - 160 cm2 surface area

KrosFlo® TFDF® Pilot System

1 - 500 L volume
500 - 1600 cm2 surface area

KrosFlo® TFDF® Process System

50 - 2000L volume
3000 - 6000 cmsurface area

READY TO SCALE FAST

Capacity increases with tube number and length

To ensure linear scalability from 1 to 2000 L, the tube length and the number of tubes per enclosure increases. Tube length increases from 20 cm to 108 cm. The effective length of the filter may be less than the physical length of the filter. The number of tubes increases from 1 - 40 per enclosure.

Number of tubes

1 tube
Surface area: 3 cm2
Recommended Volume: <1 L

1 tube
Surface area: 150 cm2
Recommended Volume: <50 L

10 tubes
Surface area: 1500 cm2
Recommended Volume: <500 L

40 tubes
Surface area: 6000 cm2
Recommended Volume: <2000 L

* additional sizes available 

 

Length of tubes

Physical length: 20 cm
Effective length: 2 cm
Surface area: 3 cm2

Physical length: 108 cm
Effective length: 108 cm
Surface area: 150 cm2

KrosFlo® TFDF® Systems

The KrosFlo® TFDF® System delivers a complete solution for optimized tangential flow depth filtration, with integrated process control logic and a single-use, Gamma-irradiated ProConnex® TFDF® Flow Path with integrated sensors.

Click on the tabs to explore features of the different KrosFlo® TFDF® Systems and Flow Paths.

KrosFlo® TFDF® Lab System

1 - 50L volume
3 - 160 cm2 surface area

Click on the links to explore different features.

KrosFlo® TFDF® Pilot System

1 - 500L volume
500 - 1600 cm2 surface area

Click on the links to explore different features.

KrosFlo® TFDF® Process System

50 - 2000L volume
3000 - 6000 cm2 surface area

Click on the links to explore different features.

ProConnex® TFDF® Flow Path

Click on the links to explore different features.

KrosFlo TFDF Lab Flow Paths

Off-the shelf, single-use, gamma-irradiated and closed TFDF Lab Flow Paths arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.

See specifications

More information

Custom KrosFlo TFDF Pilot and Process Flow Paths

When scaling up, configure a flow path to meet your process requirements for your pilot and process KrosFlo TFDF Systems. All KrosFlo TFDF flow paths are single-use, gamma-irradiated and arrive dry, free of sodium hydroxide, glycerin, preservative and ethanol, for a zero flush start state.

See specifications

More information

The R Factor Video Blog
play icon Video poster
Espresso depth filtration

Replace sluggish messy depth filtration with combined tangential flow and depth filtration. No need to load multiple filters or hunt for parts. At 1L through 2000L, connect one fully integrated filter module and go -- at flux speeds up to 1950 LMH.

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The 3-Minute drill

If you have three minutes, then you have enough time to install a TFDF® flow path. Mount the device, make a few connections and you’re done - without ever breaking a sweat.

Manufacturing Centers of Excellence

Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.

Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands,  Germany and Estonia.

Locations

Expert Project Management

Customer First.

Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.

  • Field Application Support
  • Customer Service
  • Field Service Engineers

Have a question?

Resources

REACH Statement
View article resources.
Selected Third Party References

Process intensification for lentiviral vector manufacturing using tangential flow depth filtration
by Robert M. Tona, Reeti Shah, Kimberly Middaugh, Justin Steve, João Marques, Blair R. Roszell, Cindy Jung
Cell Press, March 2, 2023

Production of Lentiviral Vectors Using a HEK-293 Producer Cell Line and Advanced Perfusion Processing
by Michelle Yen Tran and Amine A. Kamen
Frontiers, June 14, 2022

Lentiviral vector manufacturing process enchancement utilizing TFDF® technology
by Thomas Williams, Oliver Goodyear, Lee Davies, Carol Knevelman, Michael Bransby, Kyriacos Mitrophanous and James Miskin
Cell and Gene Therapy Insights, April 2020

View publication resources.