SpectraFlo™ Dynamic Dialysis Systems
SpectraFlo™ Dynamic Dialysis Systems are the only commercially available systems for scalable dynamic dialysis from lab scale to large volume processing.
Continuous buffer flow
No manual buffer changes
Walkaway convenience
Integrated
Includes dialysis tank, pump, reservoirs, membrane
Fast implementation time
Scalable
Scale up from lab to process scale
Scale out from 1 to 6 tanks in series
Increased purification efficiency
Sealed self-enclosed design
Minimized exposure to external contaminants
Innovative mobile system
Large-scale, small footprint
Series operation conserves buffer
Dynamic Dialysis
Dynamic dialysis is an exciting new technology that utilizes fluid dynamics to increase purification efficiency and improve large buffer handling, ideal for the production of fragile proteins, viscous fluids and polymer gels, such as hyaluronic acid. These closed, high-efficiency systems offer high concentration gradient dialysis without manual buffer changes and in-process sample handling.
SpectraFlo™ Dynamic Dialysis Systems
Dynamic Dialysis Applications
Dynamic dialysis is ideal for the purification of viscous fluids and hydrogels (hyaluronic acid – HA) that cannot be filtered. Where traditional filtration methods may fail, dynamic dialysis uses flow dynamics to increase both the rate and efficiency of dialysis.
|
Applications |
Why Dynamic Dialysis is preferred |
Example |
|---|---|---|
|
Delicate samples |
Gentle separation without shear stress |
Labile proteins and intact viruses |
|
Viscous samples (Hyaluronic Acid) |
Process hydrogels that are too viscous for filtration |
Cross-linked Hyaluronic Acid for aesthetics and orthobiologics |
|
Protein refolding |
Refold proteins to native state using gradual pH |
Overexpressed proteins containing inclusion bodies |
|
High debris lysate |
Will not foul like pressure driven filtration |
Demineralized bone matrix for othopedic surgery |
|
Polymer and Biopolymer synthesis |
Low MWCO enables removal of unbound low |
Oligonucleotides and polypeptides |
|
Scale up of any static dialysis application |
Easy scale up without complex validation or |
Desalting |
Tank Sizes
SpectraFlo™ Modes of Operation
Case Study: Model of Dynamic Dialysis
This model of a GMP batch production of shear-sensitive protein shows an efficent scale-up of large volume dynamic dialysis for purification.
SpectraFlo™ Mobile Features and Benefits
Dialysis Membranes
Dialysis membranes from Repligen include biotech-grade, standard-grade and custom membranes that can be used in both lab-scale and process-scale operations.
Components
Processing Reservoirs
Designed with drain and 4 ports for recirculating or reservoirs in series
Bisectors
Cart Design
Materials of Construction
Quality Specifications
- USP Class VI biosafety requirements
- 21 CFR 177.1520
- Phthalates, BPA and DEHP free
- BSE/TSE free
Quality Management System-ISO 9001:2015
