OPUS® RoboColumn® Pre-packed Columns
LEADER OF THE PACK
OPUS® RoboColumn® miniaturized pre-packed columns are designed for automated chromatography process development and sample preparation using robotic liquid handling workstations.
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OPUS RoboColumn Pre-packed Chromatography Colums
Used in the industry since 2007, OPUS RoboColumn pre-packed columns are designed to work with robotic fluid handlers for fully automated parallel chromatography.
Up to 96 RoboColumn pre-packed columns (8 column types in parallel) can be individually arranged on a 96-well array plate according to application requirements. Resin is contained to form a bed between two filter frits and validated with consideration to resin compression requirements, to ensure high efficiency, good asymmetry and robust chromatography performance.
OPUS® RoboColumn®
internal design
OPUS® RoboColumn®
5 mm ID x 10 mm H; V = 200 µL
OPUS® RoboColumn®
5 mm ID x 30 mm H; V = 600 µL
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OPUS® RoboColumn®
96-well plate
Click and quote OPUS® PD Pre-packed chromatography columns
Configure and quote pre-packed columns for process development using this interactive tool.
When you are planning your next set of small-scale chromatography experiments, configure the columns online and obtain a quote in minutes. Simply login, select your format, volume and resin that meets your experimental design and add to cart. A quote with all details and pricing will be emailed to you within minutes.
Make your chromatography life easier.
FLEXIBILITY
Flexibility with pre-packed convenience
- Pre-packed with user-specified chromatography resin - over 300 resins
- Consistently packed with correct axial compression
- Operating linear flow-rate controlled by dispensing speed of the liquid handling workstation
- Demonstrated scale-up and scale-down due to constant residence time
- Large number of samples per time, ability to multiplex
Available formats
Internal Diameter |
Bed Height |
Column Volume |
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| cm | mm | cm | mm | mL | µL |
| 0.5 | 5.0 | 0.25 | 2.5 | 0.05 | 50 |
| 0.5 | 5.0 | 0.50 | 5.0 | 0.10 | 100 |
| 0.5 | 5.0 | 1.00 | 10.0 | 0.20 | 200 |
| 0.5 | 5.0 | 2.25 | 22.5 | 0.45 | 450 |
| 0.5 | 5.0 | 2.50 | 25.0 | 0.50 | 500 |
| 0.5 | 5.0 | 3.00 | 30.0 | 0.60 | 600 |
Minimum order is one row of 8 OPUS RoboColumn pre-packed columns with the same resin and column dimension.
Automation
Automated parallel chromatography
Shorten process development time by performing parallel operation of OPUS RoboColumn pre-packed columns in robotic liquid handling workstations, implementing chromatography Design of Experiment (DOE) and QbD (Quality by Design). The combination of OPUS RoboColumn and Tecan robotic liquid handling workstations deliver a complete integrated platform for state of the art small scale protein purification.
Designed for robotic fluid handlers
Compatitble with both Tecan Fluent and Freedom EVO liquid handling workstations
- Simultaneous automated step-gradient elution into a 96-well plate
- Subsequent analysis in an integrated plate reader
- Flexible software wizard
Rapid and simple AAV capture and polish DOE with new digital tools
This outline describes the advanced and fully automated purification development and optimization for an AAV vector with OPUS® RoboColumns® and Tecan Fluent liquid handling workstation.
High Performance
Process Development.jpg)
Development of a purification process for a specific molecule requires evaluation of the resin as well as operation conditions for each chromatography step.
- Resin screening
- Condition screening
Using OPUS RoboColumn on a Tecan Fluent liquid handling workstation generated up to 96 pseudo-chromatograms (1536 data-points) in less than one day.
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Sample Preparation / Process Analytical Technology (PAT)
The development and manufacture of a biotherapeutic requires significant analytical characterization of in-process intermediates. Non-purified samples derived from small scale multi-parallel bioreactors, such as Ambr™, requires sample preparation before analysis can be executed.
- Drug discovery and clone selection
- Protein characterization
- Media optimization
- In-process fermentation monitoring
Using the OPUS RoboColumn on a Tecan Fluent liquid handling workstation generates sufficient purified product to conduct your analytical assays.
Solutions
Manufacturing Centers of Excellence
Repligen develops and manufactures products for the biopharmaceutical industry under an ISO 9001 quality management system. We focus on the timely delivery of high quality, consistent and robust products, to ensure business continuity for our customers.
Repligen manufacturing sites are located in Massachusetts, California, and New Jersey in the United States and in Sweden, France, The Netherlands, Germany and Estonia.
Customer First.
Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.
- Field Application Support
- Customer Service
- Field Service Engineers
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Resources
Specifications
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Plate size |
128.3 L x 86.0 W x 14.0 mm H |
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Plate material |
Polyoxymethylene copolymer |
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Number of wells |
96 |
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Column volume |
50 µL |
100 µL |
200 µL |
450 µL |
500 µL |
600 µL |
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Bed height |
2.5 mm |
5.0 mm |
10.0 mm |
22.5 mm |
25.0 mm |
30.0 mm |
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Column inner diameter |
5 mm |
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Column material |
Polypropylene |
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Chemical stability |
All commonly used aqueous buffers, pH 1 – 14, organic solvents*. Avoid the use of halogenated organic solvents, hexane |
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Column storage solution |
AIEC and CIEC Columns: 20 % ethanol + 150 mM sodium chloride |
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Recommended storage temperature |
AIEC, CIEC, HIC, SEC and MMC: +4°C to +30°C |
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Working temperature |
+4°C to +30°C |
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Maximum working pressure |
Up to 8.0 bar |
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Flow velocity |
16 – 1000 cm/h** |
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Labelling |
Laser-etched on every individual column body. Label contains:
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* Note: The chemical stability of the packed chromatography resin has also to be taken into consideration. For more information please refer to the manufacturer’s resin specification.