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Cell Culture

Cell culture, or cell expansion, refers to upstream methods that enable the growth of eukaryotic or prokaryotic cells in physiological conditions, for subsequent downstream purification and filtration.

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Cell culture processes are used to produce the vast majority of protein therapeutics. A typical cell culture process starts with growing and expanding cells in suspension through a series of reactors of increasing size (also known as the seed train), until a sufficient number of cells have been produced to seed the production reactor.

Process intensification, effective cell culture media, more stable cell lines, and bioreactor control strategies aim to improve upstream productivity and biologics quality.

Repligen solutions help overcome key challenges in cell culture, with hands-on process and implementation consultation from global Field Applications Specialists.

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Cell Culture Unit Operation

Click on the workflow link below to find productivity and throughput solutions you can implement today. 
Repligen workflow solutions include hardware, software and consumables that scale from process development to large-scale GMP manufacturing levels.

Perfusion Cell Culture

Perfusion, a cornerstone of upstream continuous bioprocessing, has been practiced since the 1980s to maximize facility utilization, expand process flexibility and minimize costs. Today, novel cell retention devices and single-use technologies, with sophisticated control logic, simplify implementation.

N-1 Perfusion

A form of seed train intensification, N-1 perfusion refers to the intensification of cell growth in the seed train step (N-1) before the production bioreactor (N), achieving a more rapid and robust process while maintaining an existing Fed-Batch based production scheme.

Seed Train Intensification

Seed train intensification aims to generate ultra high-density cell cultures at optimal points during cell expansion using a robust cell retention device prior to the inoculation of the production bioreactor.

High Productivity Harvest

High Productivity Harvest (HPH) is a novel application developed by Repligen that enables the harvest of a Fed-Batch bioreactor in a fully sterile manner ready for processing on a capture column, eliminating both centrifugation and depth filtration.

Continuous Manufacturing

FROM BATCH TO CONTINUOUS

Continuous manufacturing is a method for manufacturing pharmaceutical products from end-to-end on a single, uninterrupted production line. The aim is to eliminate batch inefficiencies that result in process stoppages, long hold times, and sub-optimal facility utilization.

Fluid Management

Repligen offers innovative, designed-for-purpose storage and transfer solutions for fluid management within and between unit operations. These include flow paths, tubing, valves and gaskets, bottles and containers, totes, carts and cleanroom process equipment.

Customer First.

Support is part of the Repligen DNA. Our goal is to provide exceptional customer experience, and to support the efficient and successful adoption and implementation of all Repligen products and services.

  • Field Application Support
  • Customer Service
  • Field Service Engineers

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