CTech™ SoloVPE® PLUS System
UV-Vis Concentration Measurement for GMP and Non-GMP Testing
The new CTech™ SoloVPE® PLUS System features patented variable pathlength technology (VPT), providing precise concentration measurements with no need for costly dilutions. This reliable solution delivers fast, accurate results with system-to-system, operator-to-operator, and site-to-site consistency, ensuring high-quality performance across various bioprocessing applications.
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No Dilutions
Measure highly concentrated samples directly without dilution.
Eliminate Background Correction
Buffer/ background correction only required in special circumstances.
Reduce Sample Prep
Direct measurements of highly concentrated samples saves time and consumables.
Rapid Results
Concentration results in about one minute.
Low Sample Volume
Method and sample vessel dependent ranging from <20 μl to 2 ml can be reclaimed.
Platform Technology
Confidently work system-to-system with +/- 2% repeatability.
Unlike the single value dependence of legacy UV-Vis methods, the data-dense slope method characterizes samples by collecting multiple absorbance data points at several pathlengths to create a section curve (absorbance vs. pathlength plot).
The section curve is then analyzed in real time to verify linearity in compliance with the Beer-Lambert law. The linear region of the section curve is directly proportional to the concentration of the sample based upon the sample extinction coefficient.
The result?
The SoloVPE PLUS System reports concentration results in less than 30 seconds. Capable of making spectral and fixed-point measurements at wavelengths between 190 nm and 1100 nm at pathlengths between 2 microns and 15 millimeters, the SoloVPE PLUS solution is adaptable to a wide range of sample types and concentrations. The variable pathlength spectroscopy method has been proven to be accurate, precise, and robust. Therefore, it can easily and successfully be validated in accordance with ICH Q2(R1) requirements.
The variable pathlength technology allows R2 values of ≥0.999, proving the robust system-to-system consistency.
Slope Spectroscopy Methods: The first and only slope spectrometer, capable of Slope Spectroscopy-based measurements. The SoloVPE PLUS System takes advantage of slope results based upon multiple data points instead of a single absorbance value.
Rapid Sample Characterization: Rapidly characterize samples at different wavelengths and pathlengths using a single method.
Education/Support: On-site installation and training included with system purchase.
Linear Range Finder Technology: The system automatically identifies the linear region of section data sets to verify compliance with the Beer-Lambert law.
Dimensions: 257 mm x 245 mm x 464 mm (10.1 in x 9.7 in x 18.3 in); includes Strain Relief
Weight: 20 lbs. [9 kg]
*Spectroscopic Engine: Agilent Cary 60 Spectrophotometer Fiber Optic
Integration: CTech Dual-Use Fiber Optic Coupler
SoloVPE Power Requirements: 100–240 VAC, frequency 47–63 HZ
Cary 60 Power Requirements: 100-240 V AC, Frequency 47-63 Hz
Maximum Pathlength: 15.000 mm
Minimum Pathlength Step: 0.002 mm
Variable Pathlength Speed: >1.3 mm/sec
**Slope Repeatability: ≤ 2% RSD
Sample Vessel Compatibility: Fused silica (large, small, micro), disposable plastic (small)
Fibrette® Optical Component: OF0002 (Silica + Polyimide)
Sample Volume Required: Dependent on sample vessel used and method pathlength range
Proximity to Cary 60: On top of or within 0.5 m
‡Measurement Pathlength Axis Orientation: Vertical
Required Computer Hardware per the Minimum Requirements of the Agilent Cary WinUV Software Package:
- Min Processor: Intel i5
- Min Hard drive: 250 Gb (SSD Preferred)
- Min RAM: 8 GB
*Photometric performance characteristics are based upon the Cary 60 spectrophotometer specifications and are applicable to the Cary 60 independent of the SoloVPE System.
**Repeatability performance requires properly validated method and controlled homogeneous samples.
‡Samples that are not homogeneous, suspensions, improperly mixed, or not in solution could produce unexpected results. This should be assessed during method development.
Protein A280; Monoclonal Antibodies
- 0.01 mg/mL to 300 mg/mL without dilution
- 0.1% to 5% stock solution direct measurement
- Directly measure sample and reduce the risk of false negative results
Quantitation of Nucleic Acids (DNA/RNA)
- Up to 200 mg/mL without dilution
- Plasmid Purity
- Detect DNA and Protein at the same time
Operating System: Microsoft Windows 10 compatible
Software Environment: CTech™ ViPER® ANLYTX software platform
VPT Software Control: ViPER ANLYTX Software
Validation Companion (Optional): ViPER Validate VPT application for use with the SoloVPE Validation Cuvette Adapter (SVCA)
Security Companion (Optional): ViPER SecureVPTsettings (for GxP implementations)
Prior Versions: Prior software versions may be available on request. Contact Sales for more information.
Legacy Support: Support for legacy products can be secured but subject to limitations.
Support Services
Enjoy the benefit of year-round technical support, scheduled maintenance, and more. We offer one- to three-year service contracts for your SoloVPE PLUS System. Schedule a visit from a Repligen process analytics representative to qualify your SoloVPE PLUS System.
Application Service
Supplementary to our standard support options, application services provide you with increased access to our highly knowledgeable and experienced professionals. We offer implementation, method development, and educational guidance to maximize the benefits of your variable pathlength solutions and the Slope Spectroscopy® technique.
CTech™ Variable Pathlength Technology Consumables
The quality of our Fibrette® Optical Components, sample vessels, instruments, and our service is unmatched.
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