KrosFlo® RS TFF Systems
The KrosFlo® RS are a series of tangential flow filtration (TFF) systems engineered for an era of new modalities. These systems are single-use and cGMP compliant with features like end-to-end automation, minimal hold-up volumes, and effortless scalability. Support automated ultrafiltration and diafiltration (UF/DF) processes for batch volume ranges of 35mL to 2000L.
WHEN EVERY DROP COUNTS
Minimize hold-up volumes and reduce batch variation with valve block technology and end-to-end automation.
ENHANCED PROCESS CONTROL
Experience intelligent mixing and recipe customization with Tulip tank and intuitive recipe-driven software platform.
SCALABLE SOLUTION
Scale effortlessly from 1/8” to 1” while maintaining process parameters.
Bench scale TFF meets cGMP production
Introducing the KrosFlo® RS 10
Meet the first bench scale TFF system built specifically for cGMP production with true end to end automation.
The new KrosFlo RS 10 takes all of the components, controls, and capabilities of our highly compliant, commercial manufacturing large-scale TFF systems and brings it down to bench scale.
One feature unique to the RS 10 is the ability to choose your own feed pump upon ordering to better fit your process needs.
- Centrifugal
- Diaphragm
- Peristaltic
Automation Redefined
While many traditional single-use TFF systems claim to be “automated” they often require frequent manual intervention by operators due to the limited number of controlled valves available. The more controlled valves throughout the system’s flow path, the greater the degree of automation capability.
With significantly more controlled valves than any other TFF systems on the market, the KrosFlo® RS enables users to truly automate the entire process, end-to-end, significantly reducing the risk of human error and inconsistencies from batch-to-batch. Automation beyond UF/DF- that’s end-to-end.
- WFI/Buffer Flushing
- Integrity testing
- Fed-Batch Concentration
- Product Recovery
- Clean-in place
- NBP testing
Recovery Maximized
Traditional hose barb assemblies found on most TFF system flow-paths space valves far apart increasing total hold-up volume and creating dead-legs which limits concentration capabilities. The ProConnex® flow paths featured on KrosFlo® RS are made up of injection molded tubing connections that fit precisely within the recesses of the system’s valve blocks.
The valve block technology allows the RS to cluster a set of valves close together for automated and optimized concentration.
These features and more make these the TFF system of choice for mRNA and gene therapy manufacturers.
Full Process Control Unlocked with KlariFi™
The RS series is powered by the KlariFi™ system control software, an intuitive recipe-driven platform that unlocks the full potential of your TFF run. Use the point and click recipe editor to build from scratch or to customize from an extensive library of pre-built templates.
Enjoy ease of navigation with a flow diagram-based interface that mirrors your system’s hardware. Process controls, settings, and data are available at a glance with the ability to visualize, pause and adjust during a run without compromising batch integrity. Best of all, KlariFi™ is built to enable CFR 21 part 11 and GAMP 5 compliance.
- Intuitive point and click interface
- Windows-integrated access control
- Transfer recipes between systems
- Secure batch reports
- Customizable data trending
Intelligent Mixing Even at the Lowest Working Volume
Innovative tulip tank technology delivers hold-up volumes as low as 1.5L with active mixing.
As fluid levels drop, the dual impellers independently adjust speed to reduce harmful surface interaction while automated valves adapt retentate flow to avoid damage upon entering the tank.
Tulip Tank cart available with KrosFlo® RS 30, 40, 50
Filter Flexibility
Expand your application usage by using the KrosFlo® RS with flat sheet cassettes or hollow fiber filters. Swap between filter types in less than 10 minutes.
Repligen offers the broadest range of membrane formats in the market with TangenX® Cassettes and Spectrum® Hollow Fiber filters.
KrosFlo RS 30 TFF System Overview Video
New to the KrosFlo RS Systems? Get an immersive overview of the KrosFlo RS 30 TFF System, Repligen’s integrated solution to empower cGMP manufacturers with end-to-end automation and optional real-time concentration control. This video will explore topics such as:
- System Introduction
- Valve Block Technology
- Mixing Vessel
- Process Flow Overview
- In-line Protein Concentration Control
- Software Overview
- Network Integration & Compliance
Scale up Streamlined
Designed as a single standardized platform and not as standalone systems, KrosFlo® RS share the same industrial grade components and automation capabilities across the entire system series. With the same software, hardware, controls and cGMP compliance built into every system, challenges in scale-up are significantly reduced, allowing you to confidently maintain your process as you scale.
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| System |
RS 10 |
RS 20 |
RS 30 |
RS 40 | RS 50 |
| Tubing Diameter | 1/8" | 1/4" | 1/2" | 3/4" | 1" |
| Flatsheet | 0.01 - 0.1 m2 | 0.1 - 0.5 m2 | 1 - 5 m2 | 1 - 10 m2 | 5 - 20 m2 |
| Hollow Fiber | 0.02 - 0.6 m2 | 0.05 - 1.0 m2 | 0.05 - 5 m2 | - | 4 - 34 m2 |
| Recirculation Pump (Diaphragm) |
0.5 LPM | 3.4 LPM | 20 LPM | 41 LPM | 83 LPM |
| Centrifugal | 1.15 LPM | - | - | - | - |
| Peristaltic | 0.34 LPM | - | - | - | - |
Upgrade to RPM™ for Real-time Concentration Control
Integrated with the CTech™ FlowVPX® in-line spectrophotometer, unlock advanced analytical capabilities based on real-time concentration data. Reduce variability, offline sampling and dilution steps.
- Greater control and reproducibility
- Increase efficiency and yields with reduced cycling
- Reduce risk with real-time concentration data
Explore the System Components
KrosFlo® RS single-use Tangential Flow Filtration (TFF) systems incorporate the most advanced engineering principles alongside unique hardware to achieve unprecedented automation, hold-up volume and process control.
Click on the tabs to explore features of the different KrosFlo RS Systems.
KrosFlo® RS 10 TFF System
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KrosFlo® RS 20 TFF System
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KrosFlo® RS 30 TFF System
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KrosFlo® RS 40 TFF System
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KrosFlo® RS 50 TFF System
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Error-free installation in minutes with pre-assembled flow paths
ProConnex Flow Paths, pre-assembled and specific to the KrosFlo® RS TFF System, are intuitively designed for simple, mistake-free installation. All system sensors are pre-calibrated and assembly is done in minutes with no confusing instructions.
Click on the left/right arrows or swipe to see flow path assembly steps for the different KrosFlo® RS TFF Systems.
Related products
ProConnex® Flow Paths for Systems
Expertly designed plug-and-play flow path
ProConnex® Flow Paths for TFF and Chromatography processes are single-use and engineered for optimum performance, reproducibility and time savings. System specific flow paths provide speed and reliability. Configured flow paths prioritize process requirements and sterility.
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